Supreme Pharmacist System

Institute of Cryptococcosis, No. 2333, Jiangzhou Science and Technology Park.

It has been more than half a year since its establishment last year.

But so far, the institute has found nothing.

The reason has already been mentioned before, cloning cells is too difficult, because the content of antigenic B cells in serum is too low.

After the B cells are isolated, the sensitivity and specificity requirements for their sequence amplification and determination are also very high.

Even if the sequence is amplified and the antibody is expressed, the "neutralizing effect" of the antibody needs further identification.

In short, it is extremely difficult to replicate antigenic B cells.

Of course, the above is for the average person.

Zhou Wen is not included.

He has now upgraded to LV4 in several subjects, and he can almost start trying to do this task.

This mission brought Zhou Wen not only experience, but also other additional things, such as influence in the academic world, which other missions could not bring him.

On the afternoon of May 5th, Zhou Wen officially started cell cloning research.

The first step is to isolate the B cells.

This is extremely difficult for others, but for Zhou Wen, who has the eyes of true seeing, it is a piece of cake.

First, his eyes can directly see the antigenic B cells in the serum;

Secondly, the upgrading of biological immunology to LV4 brought him not only a simple amount of knowledge, but also a deeper understanding of antigenic B cells. He mastered a set of unique separation methods.

These techniques are independent of existing biological knowledge.

A week later, Zhou Wen successfully isolated cryptococcosis antigenic B cells.

Li Feng and Fan Bo, who had been complaining about Zhou Wen in their hearts, nearly popped out of their shocked eyes.

The other 13 doctoral researchers in the laboratory were also shocked.

Everyone's admiration for Zhou Wen is like a surging river,

Continuously, like the flood of the Yellow River, out of control.

But everyone is not cheering!

This is just the first step in the Long March.

Next is the main event - "sequence amplification"!

This is extremely difficult for ordinary people, because antigenic B cells are too rare, and the failure rate is as high as 99% during sequence amplification!

And if you fail, you may never have another chance.

But this is not a big problem for Zhou Wen.

Don't forget, how did Zhou Wen start his business, DNA extraction and amplification!

He thinks he is second in extracting DNA and PCR all over the world, and no one dares to claim the first.

Of course, Zhou Wen didn't dare to take it lightly, so he cheered up and went all out.

Because there are too few specimens.

If it fails, the isolated B cells will be useless, and it will take a week to re-isolate, and it may not be a one-time success!

It is similar to the mouse DNA amplification procedure, except that he redesigned the primers himself.

Several simulation experiments were also carried out.

Six days later, Zhou Wen successfully expanded a sufficient amount of antigenic B cells, and after product analysis, it was determined that the expansion was successful!

Now there is no shortage of research samples.

The whole lab is boiling!

Everyone looked at Zhou Wen as if they were gods.

If it is said that the one-time isolation of antigenic B cells is still in the human category, then the one-time expansion of antigenic B cells without operating experience and without the opportunity to repeat failures is completely non-human technology.

At this moment, Zhou Wen has become their spiritual belief!

Even if he says he is an alien now, they still believe it!

Next came the antibody expression assay.

Antibody expression is a multi-step process that begins with transcription from DNA to mRNA and culminates in translation of mRNA into protein…

A 30,000-word introduction is omitted here.

Not surprisingly, the sensitivity of antibody expression is naturally a one-time pass.

Li Feng and a group of other doctors were not surprised by this at all, and even took it for granted in their hearts.

The next step is the most important step, and the last step is to test the "neutralization effect" of the antibody.

Because the "antibody" itself only has the function of recognizing the target antigen, and does not have the function of killing or rejecting the target antigen.

And the "neutralization effect" is that the combination of antibody and antigen produces a precipitate or cell group, which is then swallowed by phagocytes, and then the antigen is completely killed!

If the neutralizing effect test is successful, it means that the vaccine for cryptococcosis has been successfully manufactured.

...

Xiangya Hospital, Director of Infectious Diseases Office.

Cheng Yanqing was writing an experiment report when the phone on the desk rang.

She picked it up and took a look. It was Pan Renfeng, a member of the Virus Exchange House and director of the Department of Infectious Diseases at Xiehe Hospital.

"What's the matter, Director Pan?"

"Zhou Wen said that the antigenic B cells they amplified have been tested for antibody expression, and the neutralizing effect test will be carried out soon."

Cheng Yanqing was stunned for a moment, then turned pale with shock and said, "What did you say?"

"I said that Zhou Wen and the others have isolated and amplified the antigenic B cells of cryptococcosis, and the neutralization effect test will be carried out next."

"Impossible!" Cheng Yanqing said categorically, "I called the research institute at the beginning of the month, and it is still doing basic research there. It is impossible."

Pan Renfeng said: "I didn't say this, it was Zhou Wen himself. You can check it out in the group."

Cheng Yanqing heard the words and immediately opened the WeChat group, and finally saw Zhou Wen's speech.

It was the Xia family's old house who asked Zhou Wen what he was doing recently, and Zhou Wen casually replied that he was cloning antigenic B cells for cryptococcosis.

Then Xia's old house asked him how his research was going?

Zhou Wen said that the neutralization effect test will start immediately.

Zhou Wen's words blasted out all the bosses in the group who were diving and spying on the screen.

Everyone couldn't believe it.

But after Zhou Wen posted a few test data sheets, the group immediately exploded.

Cheng Yanqing clicked on the test data and looked at it, and was beyond shocked!

She wondered if today was April Fool's Day?

How could such a thing exist?

She called Li Feng at the beginning of Mingming, and he sighed and said that there was no progress, and his words revealed complaints about Zhou Wen.

But now...

Cheng Yanqing immediately called Zhou Wen, who was on the phone.

Cheng Yanqing did not hang up the phone, but kept waiting.

After about tens of seconds, the call was connected.

"Zhou Wen, what's going on?"

"That... I can't explain clearly in a while, so how about it, Director Cheng, if you have time, come to Jiangzhou tomorrow and talk face-to-face."

Cheng Yanqing had too many questions in his mind to ask, but right now he didn't know where to start, so he had to hang up the phone for now.

How could Cheng Yanqing wait until tomorrow to go?

Without saying a word, he immediately set off for Jiangzhou.

On the way, he called Li Feng, the head of the laboratory, over and over again.

Finally got through.

Under Li Feng's narration, Cheng Yanqing's jaw almost dropped from shock, and he couldn't recover for a long time.

"Beep beep——"

Finally, Cheng Yanqing was awakened by the sound of the horn behind him, only then did he realize that he had stopped at the intersection of traffic lights.

Cheng Yanqing took a deep breath after starting, and asked, "Why didn't any of you tell me about such a big matter?"

Li Feng said: "Because Zhou said that the process is not important, but the result is important. If the neutralization effect test fails, all the sweat and hard work before are meaningless, so there is no need to let everyone know."

Cheng Yanqing: "..."

Is this speaking human language?

What a feat it is to successfully isolate antigenic B cells, and it also has a very important reference for the isolation of antigenic B cells in other rare diseases!

In her opinion, this technology alone is worth three Lancet articles!

How can it be said that it is meaningless?

As for the subsequent sequence amplification, it is also very important.

"This little guy is really outrageous!"

After Cheng Yanqing said something, he couldn't help laughing.

...

May 26, 2019, Sunday.

At 9:30 in the morning, the Jiangzhou Institute of Cryptococcosis carried out a test of the neutralization effect of antigenic B cells of cryptococcosis.

Some good friends and leaders who are familiar with Zhou Wen visited the site.

Like Pan Renfeng, Cheng Yanqing, Gu Yunwen, Mu Yuanqing, Lin Huaidong, Zhang Wen, as well as several leaders of Jiangzhou Medical College and highly respected experts and professors.

The results were not surprising.

Under the attention of everyone, the cell group produced by the combination of cryptococcosis antibody and cryptococcosis antigen was swallowed up by phagocytes bit by bit!

"Papa papa—"

In the observation room outside the laboratory, there was warm applause!

"Congratulations, Director Cheng, you are really amazing."

"This is an amazing invention! Director Cheng, you guys are doing a great job!"

"..."

Facing the praise from a group of old friends, Cheng Yanqing laughed and said: "It's alright, alright, you guys don't brag here! I'm just the leader, and all the research work is done by Zhou Wen, which has nothing to do with me .”

The experts at the scene knew what was going on, so everyone smiled.

It doesn't matter if you joke around among friends, but they are not so cheeky when they really want to rob other people's academic achievements!

And this can't be taken away at all.

Several core technical processes in it, if the experimental restoration cannot be done by then, the embarrassment will be thrown to grandma's house.

The experts were embarrassed, but one of the leaders of Jiangzhou Medical College laughed and said, "Director Cheng is too modest!

It is undeniable that Xiao Zhou's ability is outstanding, but we can't ignore the role of leadership. Without leadership guidelines and good scheduling, their young people can only wander around like headless chickens, right? "

Seeing the leader's serious face, Cheng Yanqing was really embarrassed, and said to himself: "I'm not humble, please brag and don't take me with you!"

Seeing the expression on Cheng Yanqing's face, Pan Renfeng, Lin Huaidong, Zhang Wen and others couldn't help laughing.

But the potbellied leader of Jiangzhou Medical College was baffled in his heart, and said to himself, "Are my words funny?"

But he didn't dare to question, let alone put on a face.

Pan Renfeng, Gu Yunwen, Lin Huaidong, Zhang Wen, etc., which one is not a bigwig in the domestic medical system?

That is to be able to talk directly to the chief executive.

At his level, it's not enough to see...

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