Supreme Pharmacist System

Due to the particularity of prions, Zhou Wen was extra careful when conducting experiments.

Biological masks, biological gloves, biochemical protective clothing, fully armed.

The four of Qin Haitao and the others were just helping out, and did not directly touch the container containing the virus.

There are currently four methods for detecting prions.

1. Electron microscopy.

2. Capillary SDS-gel electrophoresis.

3. Capillary electrophoresis immunoassay of fluorescently labeled peptide chains.

4. Two-color strong fluorescent target scanning method.

Among them, the second "gel electrophoresis method" is the best and most direct detection method.

Mainly through physical and chemical methods - differential speed, ultracentrifugation, sodium N- (lauroyl sarcosine), PK treatment, purify PrPsc.

Capillary SDS-gel electrophoresis was then performed on a "capillary electrophoresis machine".

According to the time and peak shape of the absorption peak at 214 nm wavelength when each component passes through the detector, PrPsc, the pathogenic factor of prion, is identified.

This method uses less specimens and does not require immunological reagents, so the judgment is very intuitive.

The disadvantage is that the two equipments of "ultracentrifuge" and "special capillary electrophoresis instrument" are needed.

At present, ultracentrifuges are basically purchased from foreign brands, and the price starts as high as 500,000 RMB.

The special capillary electrophoresis instrument for prions is also very expensive, requiring more than 200,000 RMB.

Even if it is expensive, the key is that there are few opportunities to use it.

Ordinary hospitals can't even make money back from purchasing equipment, and only the wealthy third-level hospitals like Jiangzhou Hospital will purchase it.

Because of this, medical resources in big cities are scarce.

A small city has a strange disease, and you can't even do a test.

According to the physical and chemical methods, Zhou Wen carefully prepared the reagents, extracted the diluent step by step, and put them in an ultracentrifuge to separate the mutated prion protein.

The newly upgraded biological immunology made him more handy.

During this process, Zhou Wen was also teaching Qin Haitao and Dong Wenying what to pay attention to during routine prion physical and chemical experiments.

"When adding lauroyl, you must remember..."

These experiences, not to mention university professors, even experts from domestic prion research institutes can't explain clearly.

Because domestic research on viruses, especially prions, is basically still in its infancy, these practical experiences can only be mastered by top research institutes abroad.

For example, the Yeast Prion Institute at the University of Kent, UK.

For a long time, new discoveries in the field of prion proteins have almost been "monopolized" by several research teams in the field of yeast.

Those top research institutes will not easily teach you technology.

Unless you can get their offer.

And Qin Haitao and the others obviously didn't realize how lucky they were, they just felt that Zhou Wen's explanation was very easy to understand, and even told them the essentials of the movements.

When Zhou Wen spoke, both Qin Haitao and Dong Wenying listened attentively.

As for the other two old experimenters, although they were listening on the surface, they actually didn't listen at all.

Because they feel that it is impossible to extract prions with Zhou Wen's method, and this kind of teaching is basically just talking on paper.

Zhou Wen didn't have an "electric eye", how could he know these specific experimental variables, everything was just his guess.

After the PrPsc was purified, Zhou Wen injected the serum containing prions into Beckman P/ACE 5 500 for electrophoresis experiments.

"Okay~" After the injection was completed, Zhou Wen heaved a sigh of relief.

"Qin Haitao, stay and take care of the machine, and the others wash their hands and eat."

Everyone heard the words,

Go to the sink to wash your hands...

After eating, Zhou Wen didn't dare to go anywhere, and went straight back to the laboratory.

The samples obtained by the conventional extraction method are too rare, and it is really worrying not to look at them personally...

At 7:30 in the evening, the electrophoresis experiment ended.

One of the experimenters, Huang Xingzhong, casually glanced at the electrophoresis analysis sheet, subconsciously thinking that this experiment would fail like before.

However, in the next second, a look of surprise appeared on his face, "Hey, come and take a look, it seems that prions have been detected..."

The rest of the people gathered around after hearing the words.

"Hey, it really is."

"Teacher, come and see, we have detected prions."

"Hiss...it shouldn't be, how is this possible, is it a mistake..."

Zhou Wen took the analysis sheet and looked at it carefully, and soon his face showed an uncontrollable joy.

No matter how well the previous experiment was done, nothing is 100% until the results come out.

For example, if the capillary electrophoresis instrument fails, it will also cause the detection to fail.

This will make all previous efforts all in vain.

So sometimes when doing experiments, luck also plays a part.

After confirming that it wasn't dizziness, everyone showed indescribable excitement on their faces.

To be honest, none of them, including Qin Haitao and Dong Wenying, believed that prion protein could be extracted by conventional methods.

It is really a matter of great pride to extract prion protein by conventional collection methods without hope.

After the excitement passed, Qin Haitao immediately notified Yang Liqun and Zhang Shuguang.

After hearing the words, the two rushed over immediately.

"According to the test, this is aggregate prions..."

Yang Liqun and Zhang Shuguang looked at the clear electrophoresis analysis sheet, their faces were full of surprise, joy, excitement, excitement and disbelief.

"Is this really detected in the urine?"

"It's the Zhang group! The samples were collected by Zhou Wen and I."

Hearing Dong Wenying's words, Yang Liqun and Zhang Shuguang looked incredible.

In the case of routine detection and unconventional detection, the meaning of extracting prions is completely different.

This is a very important discovery with high research value.

"You are all good. Although this is only a small step for prion research, it is a big step for China in the field of prions."

"Thank you for the praise from Group Yang. It's mainly because of Mr. Zhou. If he hadn't been persistent, we might have given up at first glance."

Yang Liqun and Zhang Shuguang looked at Zhou Wen.

The two of them have always been very optimistic about this young man, and Zhou Wen has lived up to expectations. He has shown a very strong talent in biomedicine.

And it often brings them some surprises.

Like this time.

They also predicted yesterday that it is absolutely impossible to extract prion protein under conventional collection methods.

As a result, Zhou Wen was "slapped in the face" the next day.

But this face is comfortable.

If possible, they hope that he will continue to hit the face, preferably left and right!

After being excited, Yang Liqun and Zhang Shuguang went to the computer to analyze and study the sample collection records, hoping to find certain patterns from them.

Zhou Wen also admired the actions of Yang Liqun and Zhang Shuguang.

This is the spirit of scientific research.

Although no one wants to collect prion protein in a routine state, it cannot be ignored because of not wanting to study it.

Only by knowing which factors will lead to the excretion of prion protein through body fluids can better prevention and control be carried out.

The next morning, Yang Liqun reported Zhou Wen's discovery to the hospital, requesting to formally set up a "Prion Research Team" under the testing center.

The leaders of the hospital held a special meeting to discuss whether to approve the establishment of this research group?

As the First People's Hospital affiliated to Jiangzhou University, it is not too bad in terms of hardware equipment or talent pool, but why is it not as good as other hospitals?

The main reason is that Jiangzhou No. 1 Hospital knows everything and knows everything, and there is no "unique skill" that can be used.

For example, the Jinling Military General Hospital in the province is well-known throughout the country in terms of kidney disease.

For example, Huashan Hospital in Zhonghai is very good at dermatology, infection, and neurology.

Another example is the First Hospital of Kyoto University, which is invincible in urology.

And the Orthopedics Department of Changzheng Hospital...

As for the four celestial regiments, Beixiehe, Nanxiangya, Dongqilu, and Xihuaxi, they are at the level of immortals, and even Jiangzhou Hospital can't keep up with them.

In short, Jiangzhou Hospital can be compared with other municipal hospitals except Jinling in the province, but it is not worth mentioning in China.

Because of this, Jiangzhou Hospital is in urgent need of the same medical skills that can be used to "suppress the place".

In the end, the research team's application was unanimously approved.

Because of Jiangzhou City...it should be said that the entire Jiangsu Province, there is no authoritative expert on prions.

As the initiators of the virus research, Yang Liqun, Zhang Shuguang, and Zhou Wen undoubtedly became the backbone candidates of the research team.

Under the recommendation of Yang Liqun and Zhang Shuguang, Zhou Wen became the team leader of the [Prion Research Team] of the testing center, and the two of them were honorary deputy team leaders.

The first group members: Qin Haitao, Dong Wenying, Huang Xingzhong, Liu Shan...

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ps: Sorry, please...