Rebirth of the God-level Xueba

. The active contact of the Americans, especially Dalbeco, gave Zhang Leshan and other five groups a great stimulus. (from Q pen i house u)

Zhang Leshan and others, in order to welcome the arrival of the Americans, did not hesitate to increase the funding for PCR-related experiments.

For example, Director Zhao's heat-resistant polymerase group even increased expenditure and manpower in order to improve the speed.

The biology department of the entire faculty is showing a thriving situation. Every time Dean Zha comes out of the office to inspect and sees the vitality of the newly established laboratories under his subordinates, he is deeply relieved. He only feels that the entire scientific research system seems to be To be brought alive by yourself.

Director Zhao and others also felt very refreshed. Compared with doing research by themselves, repeated experiments or reverse engineering always make people feel more confident.

Innovation is like a deep mountain and old forest that no one has ever been to. If you drill into it, you may not get anything. out of possibility.

Repeating experiments or reverse engineering is easy. It's like traveling to a famous mountain and river. You can basically determine what you can get when you get in. The only thing you need to do is to work hard, maybe I have to give some more tickets, that's all.

Compared with the deep mountains and old forests, it is natural that the famous mountains and rivers developed by the predecessors are more comfortable. If you can save tickets and travel expenses, that would be even better.

Director Zhao and the others' refreshment lasted at least half a month. By mid-February, Director Zhao's own heat-resistant polymerase group first had problems.

"The high temperature resistance of the polymerase produced according to the literature can only reach 45 degrees at most, and it cannot be raised any further." Zhu Guanghui, the researcher in charge of the experiment, reported to Director Zhao in a state of desperation.

"Didn't I ask you to do it again?" Director Zhao, who just came back from Donglaishun, was full of oil, and didn't care about tasting the taste.

"I made it again, and re-isolated the strain, but after making it, the high temperature resistance has... increased by two degrees." Zhu Guanghui spoke quickly and anxiously, and only lowered his tone when he mentioned the specific temperature. Fast, but also anxious.

Director Zhao frowned, and after a while, said: "45 degrees is too much difference."

Zhu Guanghui blushed a little, and immediately puffed up his chest and explained: "I know that 45 degrees is a little lower, but compared with our previous research on thermoresistant bacteria, this temperature has been raised a lot."

"Before Yang Rui made heat-resistant polymerases, the best heat-resistant polymerases could not reach 40 degrees. Now we have increased by at least 20%." Another researcher next to him also said.

"Yang Rui made the heat-resistant polymerase more than 90 degrees." Director Zhao only felt that the teeth were stretched tightly, as if shredded meat was stuffed in.

Zhu Guanghui's face turned red. He was afraid that Director Zhao would say this before, but now he finally heard it.

"With a little more time, we should still be able to improve." Zhu Guanghui's voice was low and thin, as if he was grilling meat.

"How much has it been raised?" Director Zhao asked.

"50 degrees should be fine, **** nice novel:."

"50 degrees doesn't make any sense." Heat-resistant polymerase is the raw material for the PCR instrument to work, and it must be able to withstand a high temperature of 96 degrees. Polymerases below this temperature are just ordinary polymerases.

If there is no heat-resistant polymerase, then you have to go back to the era of manual PCR, and do DNA replication like making spicy hot pot.

It can be said that heat-resistant polymerase is an important part of PCR instrument. It can even be said that there is a generation of PCR instrument with heat-resistant polymerase. PCR technology without heat-resistant polymerase is just a usable technology. That's all, it's called pcr0.1 at most.

Director Zhao stared at Zhu Guanghui with serious eyes.

Zhu Guanghui stumbled and said, "We still need time."

"We have time, how long do you need?" Director Zhao's expression did not soften, he knew there must be some problems, otherwise, the atmosphere in the laboratory would not be so good in the first half month, especially the half month after the official start.

Zhu Guanghui really said: "We have discovered several situations that we did not expect before. Now, we cannot determine the exact time until we break through the difficulties."

"Give you another month, what can you do?"

Zhu Guanghui whispered: "One month is not enough."

"A month and a half?"

Zhu Guanghui shook his head slightly.

"Two months?"

Zhu Guanghui's head drooped.

"Tell me a number."

Zhu Guanghui thought for a long time and said, "Half a year..."

"Can you make the same heat-resistant polymerase in half a year?"

"Maybe we can make a usable heat-resistant polymerase." Zhu Guanghui's voice became deeper.

Usable and identical are two concepts. As an enzyme, heat-resistant polymerase actually has an optimal working temperature. The so-called heat-resistant polymerase usually means that it can also play a role under high temperature conditions, that is, it can be used. However, it must play its best role under high temperature conditions. For best performance, this is very difficult.

Yang Rui has done sufficient preparations, especially with a clear direction, in order to make a useful polymerase.

But for Director Zhao's experimental group, this problem is not so simple.

However, as the most important raw material of the PCR instrument, whether the heat-resistant polymerase is good or not will largely affect the effect of the final PCR instrument.

Director Zhao said in a deep voice, "Just maybe? You were quite confident two days ago, right?"

"We didn't expect that as the temperature rises, the problems we encounter will also increase exponentially." Zhu Guanghui looked downcast.

Director Zhao said with a dark face, "Show me."

Zhu Guanghui hurriedly took out the experimental records, and then explained several key points encountered to Director Zhao.

Director Zhao is the person in charge of the laboratory, and the level of scientific research is not weak.

After watching for a while, Director Zhao found that Zhu Guanghui's judgment was correct.

"This is troublesome." Director Zhao shook the experimental records, all he could think about was the cost and time.

Although it is reverse engineering and there are references in the literature, the specific experimental procedures, materials and details are not clear, and there are still countless possibilities for crossover.

Director Zhao's laboratory is not as good as Sinovel in terms of conditions, funding, direction and guidance, and the time they put in is far from what Sinovel invested. If they can't do it, they are Of course.

Similarly, if you travel to famous mountains and rivers, if the research related to potassium ion channels is to climb Huashan, the heat-resistant polymerase is about the same as climbing Jade Dragon Snow Mountain. It is not as difficult as Huashan, but it is the same hard work and danger.

If you are well-prepared, even healthy people can climb the snow-capped mountains in this tourist area. The only difference is time and energy.

It took Yang Rui half a year to prepare, and half a year to do it. The actual operator Huang Mao is even a Maverick-level character—his current level is naturally not up to Mavericks, but his experimental ability can already be achieved. On par with the Mavericks.

Director Zhao underestimated and only dealt with Taishan, so he would naturally estimate the wrong time and cause delays.

"Director, what should we do now?" In the laboratory, a researcher asked the question that everyone wanted to ask.

In a laboratory, the biggest and most important person in charge usually doesn't have to do experiments himself, just like the biggest and most powerful executive chef in a big hotel usually doesn't take charge of the cooking.

In addition to obtaining projects and funding, the person in charge of the laboratory mainly determines the direction and solves problems, just like the executive chef mainly decides how to make a dish and what to do when encountering problems.

In addition, he naturally has to monitor time and expenses, just like an executive chef who has to limit the speed of serving dishes and limit the use of raw materials.

Usually, the director of the laboratory can eat Dong Lai Shun if he wants to eat Dong Lai Shun, but when a problem arises, he has to make a decision.

Director Zhao's mind is full of muddleheadedness.

No heat-resistant polymerase has been made in China before.

Before PCR, this was originally a not very important branch of scientific research.

Therefore, from the perspective of scientific research, it is a very good achievement to make a heat-resistant polymerase from scratch, and raise the temperature of the heat-resistant polymerase to above 40 degrees, which is enough to fill the domestic gap—— Wait, Yang Rui has already filled the gap in the country. This is probably one of the reasons why some people don’t like Yang Rui. In the past, when foreign countries made an innovative achievement and copied it from China, this was to fill the gap. Now, Yang Rui does it Innovative results have been produced, and it is useless to copy them from China.

Therefore, before a vocabulary is reinvented in the scientific research community, classic expressions such as filling the gaps in the country become difficult to use.

As far as heat-resistant polymerase is concerned, Yang Rui actually made the high-temperature-resistant polymerase raw material several years in advance, and the capital he invested in this was not available to Director Zhao.

"I didn't expect that..." Director Zhao fell into deep thought.

"I didn't expect that..." Several scientific research groups fell into deep thought one after another. For mobile phone users, please visit