"Recombinant DNA methodology led to the development of DNA or gene cloning. By the end of the 1970s, the method of inserting short DNA fragments into a plasmid or viral chromosome and then replicating in bacteria or eukaryotic dead pigs was relatively mature. , after which I will elaborate on how gene cloning is performed and why this technique has led to breakthrough changes in molecular biology."

When Yang Rui said this, he paused for a moment, and said again: "A study I did before, the paper on the structure of potassium ion channels by cloning mutant genes, was designed based on this."

The experts and professors who were a little ready to move were all silent.

It is extremely rare for domestic scholars to publish on "cell" nationwide. Yang Rui's paper on potassium ion channels is now known to everyone in the domestic biological community.

If such a thesis is given to a middle-aged scholar, it will be worshiped as a legend.

Throwing it to an academician is equivalent to repainting a golden body.

Yang Rui wrote this "cell", which naturally has a stronger impact, but Yang Rui's identity has slightly delayed its power, so that it did not explode, but slowly spread its power.

However, the classroom has always been the place where the power of teachers is fully displayed.

This is true for professors in the classroom, as it is for adjunct teachers.

Yang Rui's thesis, at this time, is enough to deter all ghosts and monsters.

At the same time, the thesis on potassium ion channels, as a high-end application of dna recombination technology, is academically difficult, which makes people dissatisfied.

The teachers who originally wanted to talk, now all lowered their heads and obediently took notes.

They are all people who have read Yang Rui's papers, and some even know about the scientific research competition between Yang Rui and Richard, so no one wants to question Yang Rui in terms of dna recombination technology.

A cell has hundreds of thousands of words, plus a single-digit icon at most. The information contained in it is something that ordinary scholars can't figure out in a lifetime. When someone is the first to make a result, ordinary scholars can only worship.

Scholars have been immersed in it all year round, and can fully understand the difficulty of DNA recombination technology, and the application of potassium ion channels to DNA recombination technology is top-notch.

If Yang Rui doesn't mention this issue, everyone can choose to ignore it, but Yang Rui brought it up, and comparing what he said before, no matter how strong a person is, he has to admit in his heart at this time: Yang Rui is qualified to say classic inheritance The learning method is backward.

Scholars took notes and thought at the same time, concentrating on the enemy.

The atmosphere in the classroom also became more dignified every minute.

Although the students couldn't hear it very well, they all seemed to dare not show their grandeur due to the influence of the people around them.

"Simply put, from the 1960s to the 1980s, the field of genetics has gone through two stages, which is what I said earlier, the era of classical genetics theory, and the era of recombinant DNA technology. But today I would like to share with you Introducing the coming third age, the Age of Ages!"

In the momentary trance of everyone, Yang Rui continued: "The full name of the polymerase chain reaction is polymerase chain reaction. I wrote the English words on the blackboard. This is a polymerase reaction invented by myself. As of last week, my paper on this technology published in "Nature" had more than 2,000 citations..."

who said this,

Yang Rui's feeling is very good: I have created an era of biology, that's it!

Come to argue!

No one dared to argue.

All the experts and professors, young or old teachers, no matter who raised their heads or bowed their heads, lowered their heads again in a brief moment of astonishment, astonishment and understanding.

What is the concept of more than 2000 citations?

The impact factor of a journal is the total number of citations of articles in the previous two years divided by the total number of articles in the previous two years. In other words, the impact factor is the average number of citations an article receives.

And a journal with an impact factor of 1.0 means that the papers in this journal have been cited an average of 1 in the past two years.

In the index, an impact factor of 1.0 is not the bottom line, and there are many journals with an impact factor of 0.5, and these journals are considered good journals in terms of the journal world.

As for the impact factor of the fascinating top journal CNS? Generally speaking, it is more than 30 and less than 40.

In conversion, under normal circumstances, the average number of citations for papers published in "Cell", "Nature" and "Science" is above 30 and below 40.

What kind of situation is not usual? This is the case when papers like Yang Rui's are published.

As a technology that is destined to rewrite the history of biology and human science, it is reasonable and unexpected to get 2000 paper citations in just a few months.

And its value in terms of citations is now equivalent to 50 CNS-level papers, 2,000 or even 4,000 ordinary-level papers.

Even compared with the excellent super-top papers of the same category, it is also record-breaking excellence.

The top-level papers also published in "Nature" have higher citations, generally 200 to 300, and it is rare to hear 400 or even 500 citations. In the field of science, this is no longer a simple doubling The problem is that there are only those biological laboratories in the world. Everyone has their own fields. It is not a theory involving a very wide range. If the number of citations reaches 100, it is already popular all over the world.

But the end point is far from 2000 citations.

This is a technology that is destined to rewrite history. Biological laboratories around the world will understand it, use it, and quote it regardless of the field.

Yang Rui stood on the podium confidently, facing the "creaking" camera, waiting for someone to debate with him.

He waited for a full minute.

For a long minute, the classroom was silent.

"Is there no objection?" Yang Rui's clear voice echoed in the classroom.

With the humility of the Chinese, Yang Rui put his own technology alongside classical genetics theory and recombinant DNA technology, and called it the third generation technology. Some people should have shouted "arrogant".

However, a minute later, no one did.

Yang Rui then waited for another minute, but still no one did it.

Yang Rui sighed regretfully and even sadly.

Although carrying 2000 citations is enough to crush any enemy in the academic world, but it is still a bit lonely to drive straight in without a single enemy.

Seeking defeat alone? Not yet.

After all, this is a lecture hall, and lecturers can express their opinions and cognitions arbitrarily, and it is not authoritative.

If it was an international conference, the person who raised his hand would probably break through the sky.

However, although the lecture hall does not have the solemnity of an international conference, it is still a formal occasion.

Yang Rui prefers his genomics to appear in front of the world in a more comprehensive manner.

Writing a book and making a statement requires information as thick as a book in order to prove yourself.

"Since there is no objection, then I will use this as a basis to talk about the research methods dedicated to genomes." Yang Rui turned around and wrote the words "genome research methods" on the blackboard, instantly detonating the audience.

Compared with Yang Ruiwei's flag, direct technical guidance is obviously more exciting for scholars.

Of course, the students didn't understand why the experts and professors around them were so excited, and they all looked at the blackboard in a daze.

Finally, Wang Dongming, who has always been bold and conceited, couldn't bear it anymore, and raised his hand and said, "Mr. Yang, where can we see the textbook for this class?"

...

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