Rebirth of the God-level Xueba

Su Xiankai and Fan Zhen were doing their own experiments, their eyes swept over Yang Rui's table from time to time.

Since they entered the "Ion Channel Laboratory", this was the first time they saw Yang Rui doing experiments seriously.

Although it is said that the person in charge of the laboratory does not have to have practical operation ability, just like an officer does not have to have strong individual combat ability, but the officer with strong individual combat ability is obviously more vulnerable to the soldiers. welcome.

Furthermore, Yang Rui is really younger, just like a young officer who is always easily provoked by soldiers.

Su Xiankai and Fan Zhenlong were newcomers, and they were recruited by Yang Rui, so they didn't dare to challenge Yang Rui's authority, but it was okay to observe secretly.

As they observed, both of them showed obvious disappointment.

Yang Rui's operation level is too average.

Of course, this is a judgment made with high standards. Yang Rui has actually improved a lot from the previous experimental level. However, since he became the head of the laboratory, everyone's expectations for him have also increased.

For example, Xu Zhengping is a middle-aged uncle who still does not have his own ** laboratory, but his experimental level has been trained for more than 20 years. Although many movements are not correct due to incorrect learning It's not standard enough, but Xu Zhengping's experimental operation is not smooth enough after 20 or 30 years of continuous experimentation.

On the other hand, Yang Rui, even though some movements are very standard, still can't hide the jerky under his hands.

"slow."

"Too slow."

This is the common thought of Su Xiankai and Fan Zhenlong.

As for Xu Zhengping, he has long been used to Yang Rui's insufficient experimental movements. This time, what he didn't believe was Yang Rui's experimental plan.

It takes a few days to do dna sequencing, what are you kidding?

Gene sequencing technology began in 1977. At that time, there was a method of chemical degradation to determine the DNA sequence, and the dideoxy chain termination method that Yang Rui is now doing. But no matter which method is used, it is not easy to use this technology for DNA sequencing. , the error rate is high and the efficiency is very low.

Of course, no matter how low the efficiency is, it is better than the small fragment sequence method invented in 1965. The latter is like Yang Rui's potassium ion channel project. Not to mention all luck, the experiment is also extremely difficult.

Xu Zhengping believes that

In those top laboratories, some researchers who are super familiar with DNA sequencing can indeed sequence the DNA of plasmids in a few days. However, the ion channel laboratory is not a top laboratory, and it is not even a provincial laboratory. Yang Rui Nor is it a researcher who is super familiar with DNA sequencing...

In fact, there are currently no researchers in China who are super familiar with DNA sequencing.

Gene sequencing is indeed a very important biological experimental tool. If the "human gene sequencing" project is the peak of biology in the 20th century, gene sequencing can be regarded as the most important experimental tool in the history of biology.

However, throughout the 20th century, China has always been slow to beat others.

In 1965, Sanger invented the small fragment sequencing method and completed the determination of 120 nucleotides of Escherichia coli 5sta. At the same time, the country was busy burning books and beating teachers, so naturally there was no time to learn.

In 1977, the practical-level dna sequencing method appeared, and China was busy reprinting books to recruit teachers, so naturally there was no time to learn it.

As an associate professor at Peking University, Xu Zhengping only came into contact with DNA sequencing a few years ago. He only did a complete experiment once, which can be regarded as experiencing the most important technology of modern molecular biology. He probably won't do it a second time until his project involves gene sequencing.

Most domestic researchers are like this. In order to save resources, when graduate students learn these things, they also study theoretically.

Xu Zhengping knew with his cerebellum that even if Yang Rui had done DNA sequencing, it was only once and a half.

You can tell from his movements, he can be called unfamiliar, with such skills, it takes a few days to make DNA sequencing.

Xu Zhengping deeply felt that Yang Rui was at a loss, not to mention relying on this to get floating funds, these costly reagents and so on were all for nothing.

Yang Rui himself didn't notice the gazes of the people around him at all.

This experiment is indeed difficult for him and requires full concentration.

The preparation of the buffer solution and the annealing mixture can be done by assistants He Quangui and others, and even centrifugation and ethanol washing can also be done by the assistants, but when it comes time to add the solution, then run electrophoresis, or heat and cool on ice, It's all Yang Rui's work.

When Yang Rui was studying, the automatic sequencers used for DNA sequencing had reached the second generation. Automatic sequencers cannot be said to be fully automatic, but there are many more manual steps that can be replaced.

Before the government stopped, ordinary pregnant women could undergo gene sequencing and do various prenatal screenings.

But in 1984, the current dna sequencing technology did not even have the first generation. Laomei may not be able to produce the first generation of dna sequencer until next year. If the Chinese can afford it, it will take another two years - just Like an Apple mobile phone, you can afford it, but you still have to wait for the factory to produce it.

Without an automatic DNA sequencer, manual work would be complicated.

In the final analysis, DNA sequencing is to analyze the base sequence of DNA fragments. Adenine is a, thymine is t, cytosine is c, and guanine is g. These four letters circulate in various forms and constitute the basis of organisms. , and dna sequencing is to measure the sequence of these four bases.

Its principle is very simple, but it is not easy to do.

Because bases are invisible, to sequence, it is necessary to make it react, and it is a correct reaction.

Accurately reacting the required bases, step by step, is also the devil's footsteps for theology.

Yang Rui's footsteps were very slow, so slow that even Xu Zhengping couldn't stand it, but a few hours later, after Yang Rui's electrophoresis run was over, a clear gel appeared in front of several people.

"It's so beautiful..." Fan Zhenlong is older and more social. He wanted to flatter Yang Rui, but he finally found an opportunity. smile.

Su Xiankai's reaction was a little slow, and he immediately said: "It's quite beautiful..."

No matter how young Yang Rui was, when he was the head of the laboratory, the lifeblood of everyone in the laboratory was in Yang Rui's hands. From funding to publishing papers, everyone has to be controlled by Yang Rui. If you meet a better laboratory leader, you can get more funding, less boring tasks, and less trouble when the paper is published. When encountering a laboratory leader with poor character, not only the funds are low, but the results are more demanding, what's more, some laboratory leaders not only want to be the corresponding author, but even the first author, they dare to put the paper author's Sign it to people outside the laboratory, do favors or even pure transactions.

Su Xiankai and Fan Zhenlong were newcomers, and they couldn't figure out Yang Rui's temper, but at least they knew it was right to follow the hair.

Yang Rui recorded the results and continued to repeat the process just now.

This time, a few more hours later, still got a nice slab of gel and another round of compliments.

Yang Rui repeated, over and over again.

From morning to night, Yang Rui didn't stop except for eating. In the afternoon, he even added a group of experiments.

Su Xiankai and the others had nothing to praise, but Xu Zhengping's eyes were straightened.

The exact result was obtained directly every time!

In the age of manual experimentation, this is very difficult.

Anyway, this is also a fresh and brand new dna sequence. If it is a repeated experiment, it is of course much simpler to start over with other people's answers, just like the repeated DNA sequencing done by Fan Zhenlong and Su Xiankai, but the brand new DNA sequencing will always produce some unwanted answers.

It is just the last step to run electrophoresis, and you will often encounter situations where you cannot run, and then you will fall short.

Yang Rui took every step slowly, but he didn't encounter any mistakes, which made Xu Zhengping hardly dare to think about it.

But the scene that he didn't dare to think about now appeared in front of his eyes.

It's like a final exam, only one student got 100 points in the exam, and you say he plagiarized, it doesn't make sense - if it's a real exam, then there's the correct answer, Yang Rui is doing it now, but the whole world The unique s5 fragment sequencing of Tengchong thermophilic bacteria, not to mention that foreign researchers do not have Tengchong thermophilic bacteria, even if there are, they have not sequenced and published public papers. Xu Zhengping, who often checks the data, is very sure of this.

"It's really beautiful." At eight o'clock in the evening, watching Yang Rui make another clear gel, Xu Zhengping praised him sincerely.

Yang Rui smiled and said, "It's just an oil seller."

"This is a pioneering experiment of yours, it's useless just to be familiar with it." Xu Zhengping didn't believe his words.

Yang Rui actually slipped his mouth. He reversed the sequence according to the answer in his mind. Of course, it was accurate every time. Naturally, he would not refute at this time. He pretended to be modest and smiled again, saying: "Do it again group, I will rest, if you want to go back, go back first."

"Don't worry, let's take a look and learn." Fan Zhenlong casually offered another flattery.

"You all have families and houses. If you don't go back to accompany your wife, what's the point of seeing me doing experiments?"

"Moral support." Fan Zhenlong answered briefly and forcefully.

"I'll help you. My experiment is finished, and all the reagents I prepared today are used up." Su Xiankai was more direct.

Many reagents such as buffers need to be prepared immediately. Generally speaking, they are prepared when they come in the morning, and the amount of preparation is determined according to the progress. When they are used up, it is time to end the experiment. Of course, some demanding experiments can't even be stored for a few hours, so they can only be prepared occasionally.

Su Xiankai had prepared extra reagents, but he was not ready to wait until 8:00 pm. Now he wanted to continue his own experiments, but there was nothing to do.

Yang Rui gave him a seat and said, "Then stay with me for another two hours. I've finished the sequencing in the past few days. It would be nice to get tens of thousands of dollars more, right?"

"Of course, tens of thousands of dollars is fine, but it's too much to finish in a few days." Su Xiankai told the truth.

Yang Rui smiled and said, "I'm a little tired, but I should be able to finish it."

Su Xiankai couldn't help but said, "The s5 segment of Tengchong Thermophile has a total of 126 base pairs."

126 base pairs, relatively short. Yang Rui made 5 of them in one day, which is also extremely fast, but it is known that this project cannot be completed in a few days by dividing by elementary school mathematics.

In fact, this has already taken off quickly. In foreign countries, many laboratories have sequenced a gene chain, and they have tested it for a year or two. The various wonderful problems encountered during this period are too numerous to describe.

It's amazing that Yang Rui did so smoothly today, but if it's smoother and faster, Su Xiankai really can't afford such excessive flattery.

Yang Rui didn't have to explain, he just continued to do it.

No one noticed, but his proficiency has increased now.

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