Rebirth of the God-level Xueba

Yang Rui stayed in the nursing home and set up the equipment with Liu Shan.

There are many ways to isolate strains. In 1879, the French isolated high-temperature strains from the Seine River. After more than 100 years of development, the processing methods of samples have long been varied. The difference between the times lies more in the simplified methods. Improve yield.

Of course, the cost also increases accordingly.

Yang Rui first prepared a centrifuge—a machine commonly used in biology laboratories that can no longer be commonly used to centrifuge water and mud samples.

Under the action of the 10,000-times-per-minute centrifuge, the water sample will directly separate the impurities and supernatant enriched in bacteria.

The isolated bacteria are relatively large in quality, and the supernatant will also be eluted out of the bacteria after passing through the filter membrane.

They are cultured in medium again, and then they can be screened.

The principle of the mud sample is similar, but it needs to be suspended with spring water first, then centrifuged at a low speed to remove large particles, and then the water sample is used to process and enrich the bacteria.

The whole process, in theory, is very simple, but the equipment is not simple.

A centrifuge that can spin 10,000 times per minute is not cheap, and has a smaller capacity. It takes ten minutes to spin a dozen test tubes in one set, and it takes an unknown amount of time to process hundreds of kilograms of samples.

The cultivation stage is also very expensive. The agar, glucose, peptone, etc. used in the culture medium are much more expensive than the same weight of food.

In addition, the cultivation conditions of 70 degrees or even above 80 degrees also put a huge pressure on the cultivation cost.

Kang Hong helped Yang Rui transport the equipment and materials, and when he dismantled them, the last spark of competition was extinguished.

He didn't intend to engage in competition at first, and the isolation of bacterial strains is not a new technology. What is meaningful is only what strains can be isolated.

If you isolate powerful strains, you will naturally be able to invent awesome papers, but if you isolate only ordinary strains, it will be difficult to get published in introductory journals.

The results are vague like buying a lottery ticket, but Yang Rui's investment cost is so high. When Kang Hong saw it, he felt that it lacked competitive value.

"Spending so much money to isolate thermophiles? Is it worth it?" Kang Hong asked when he thought about it.

"The instrument is not bad,

After we increase the manpower, these instruments will still function. "Yang Rui answered while plugging in the wires.

Kang Hong picked up a large box of filter membranes, put them in a corner, and said, "Where are the consumables? These are ultrafiltration membranes, right? They're all imported, so you need to use so much?"

Yang Rui said "En", and said: "I originally planned to process a batch first, and then bring back a batch. These costs cannot be saved."

"It's too expensive. We can't afford it." Kang Hong clicked his tongue twice.

"If you're lucky, there might be some left." Yang Rui said casually, and then Liu Shan assembled the incubator.

There are many kinds of filter membranes, and the ones that ordinary people are more familiar with should be the filter elements in household water purifiers, which is also a kind of filter membrane, in the initial stage. These civilian products all come from laboratories, and in the 1980s, they were naturally worth a lot.

In fact, the laboratory has higher requirements for materials, and it is not cheap at any time.

In order to isolate the desired thermophilic bacteria as soon as possible, Yang Rui was very willing to spend money. Kang Hong had no reason to invest such a large amount of money, so he naturally couldn't understand it. It can only be attributed to the fact that people in Beijing University are stupid and rich.

Yang Rui had no choice but to do so.

Isolating strains of bacteria is relatively mundane research work, but isolating the desired strain is not.

Historically, most antibiotics came from the isolation of soil strains. In the past two or three decades, biologists have almost destroyed soils all over the world, and the most successful one is the terminator of tuberculosis. Waxman, who invented the systematic method of separation. Then came streptomycin, and then got the Nobel Prize.

Waksman organized as many as 50 research dogs for this purpose, spent several years, and screened out more than 10,000 strains. Got the one I wanted...

Yang Rui couldn't afford to keep 50 experimental dogs, and he didn't have a few years to waste. Fortunately, he knew which hot spring it was for sure, and as long as there were enough separates, he could always find the breed he wanted.

A simple laboratory was installed in half a day.

Yang Rui casually grabbed a handful of soil from the ground, mixed some liquid, put it into a centrifuge, and tested it.

The 10,000-rpm centrifuge made a lot of noise, but Kang Hong was elated.

"We also have one in our courtyard, which is locked with a wooden cabinet." Kang Hong stared at the machine in the transparent glass, and suddenly said.

Yang Rui understood: "The school's public laboratories are like this."

"Peking University too?" The current information is backward. The distance from Yunnan to Beijing is farther than from Beijing to Los Angeles, and it is almost as far as Fuzhou to Taipei.

Yang Rui recalled it for a while, and said: "Basically, the administrator of the public laboratory only needs the instrument to be ready, and he doesn't care whether it is convenient for you to use it or not."

"That is to say, we have to apply for a good machine at least two months in advance. If it is not done well, we will have to treat guests. After that, people will say that we have made false invoices, and what false invoices we have. All of them will be given to these grandchildren. I ate it." Any researcher who talked about the use of the equipment was full of anger.

When Yang Rui was a graduate student, he was also afraid of using instruments from public laboratories. Of course, what he was most afraid of was borrowing instruments from other laboratories.

Anyone who has been in the laboratory for half a year will have a deep understanding of this kind of pain. Yang Rui tried every means to make money and started to build his own laboratory, half of the reason was to avoid the recurrence of the predicament.

If it's just to meet personal expenses, why would he need to go to such trouble, buy a monkey ticket worth 2,000 yuan, throw it away and change a house when it's almost over, and become a billionaire in the 21st century casually.

However, just being a billionaire is not as interesting as deciding the direction of research and conducting experiments by yourself.

If there is no huge pressure and personnel struggle, as the head of a top-notch laboratory, doing the scientific research you want to do, and actually doing it, it is quite a fulfilling thing.

Kang Hong didn't have Yang Rui's experience, and he didn't know how to get rid of the current restrictions. He just asked enviously: "Then where are the instruments you took out? Didn't Peking University provide them?"

"It's funded by a Hong Kong company."

"Hong Kong people are really rich." Kang Hong asked, "Where did you know them?"

"I wrote a thesis, and they are interested...Well, the centrifuge stopped, and it looks good." Yang Rui took this opportunity to leave and get busy.

Liu Shan smiled at Kang Hong and began to attack Yang Rui.

Yang Rui slowly became serious.

Isolating ordinary strains is not worth the money, but if you isolate an extreme thermophile and then isolate the heat-resistant polymerase in it, this is more or less a good paper.

"" with an impact factor of 4.0 is probably in front of it. If the goal is lowered a little bit, it must be no problem.

In theory, spending 600,000 yuan for such a paper is justified.

Although it is justifiably cost-effective, considering that it is only a necessary step for the thesis, it still does not suffer.

Seeing that they were getting busy, Kang Hong had no choice but to leave in dismay.

Yang Rui and Liu Shan did not finish their preparations until the afternoon when the weather cooled down.

"I haven't come back yet, maybe I'm camping outside. What are we going to have for dinner?" Yang Rui looked at his watch. Camping is planned. It is likely that the round trip will not be possible.

Liu Shan tilted her head and thought for a while, then raised her hand and said, "Eat instant noodles."

"Instant noodles? Well, it just goes with hot spring eggs." Yang Rui tidied up, went to the cafeteria to buy eggs and instant noodles and came back.

At this time, instant noodles are still a new product that veteran cadres really want to try.

"Where to eat?" Yang Rui asked, and said: "I'll find a waiter and ask him to boil eggs for us."

"Let's go together." Liu Shan carried a small bag, blinked her eyes, and said, "Let's make hot spring eggs by ourselves. You can soak in hot springs, eat instant noodles, and eat eggs at the same time."

Yang Rui said in surprise: "Men and women bathe separately."

"That small courtyard is empty." Liu Shan pointed to the courtyard at the foot of the mountain not far away.

There is a sign of "Advanced Hot Spring Room" hanging at the entrance of the courtyard, which is a small courtyard built around the hot spring pool. The imported hot spring is steaming hot, which belongs to the tenants alone, and can be soaked during the day and night without using the multi-person changing room.

Yang Rui said: "They are appointed by cadres above the department level. I can't even use Li Zhangzhen's identity card."

Yang Rui has obviously tried it. The staff of the nursing home insisted on their own opinions, and Yang Rui did not insist.

After the tourism resources are fully developed, there is nothing rare in such hot springs. It is nothing more than leading out a pool of hot spring water and more pipes for water supply and drainage, which can charge a few hundred yuan more in room fees. Countless hot spring resorts have been stimulated to devote themselves to this place. .

But as far as the current state of resource development is concerned, hot springs themselves are not rare, but construction costs have become a bottleneck. In addition, the sanatoriums that develop hot springs are all fully funded by the government, and they have no urge to make profits. After the construction of a site is completed, there is almost no new investment except for repairs on weekdays.

A beautiful small courtyard with built-in hot spring is a very fresh experience in Tengchong. In the case of a small number, it is natural to keep it carefully to avoid the sudden arrival of senior leaders.

Liu Shan tightly held the small bag on her chest, and said, "There's no one inside, let's sneak in."

Then, Liu Shan took the lead, bypassed the lights at the intersection, and climbed up from the low wall behind.

The poor family built a nursing home for veteran cadres, and they didn't even consider that some residents could climb the walls.

Yang Rui looked down for a few seconds, gritted his teeth, "What are we afraid of?", and he followed suit.

Turning into the small courtyard, Yang Rui was stunned.

Under the bright moonlight, Liu Shan was stretching her waist and taking off her jacket.

In the dark, what Yang Rui saw were beautiful curves, and what he heard in his ears were delicate voices.

……(To be continued. If you like this work, you are welcome to come to the starting point to vote for recommendations and monthly tickets. Your support is my biggest motivation. Mobile phone users, please go to m to read.)

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