Plague Doctor

Zhang Lin took out the cylinder from the liquid nitrogen tank, and then took out a cryopreservation tube from the cylinder, and then put the cylinder back carefully, because there are also many cryopreservation tubes containing other cells in the cylinder. After Zhang Lin sealed the lid of the liquid nitrogen tank again, he began a series of patient and meticulous operations.

He first put the cryotube in a water bath, thawed quickly with hot water at 37 ° C, then changed the centrifuge tube and centrifuged it for five minutes, then sat down on the ultra-clean workbench, put the centrifuge tube tube mouth toward the alcohol lamp Bake and sterilize, discard the waste liquid, add fresh culture liquid with a pipette, and mix repeatedly by pipetting ...

Gu Jun has been watching carefully next to him. If he has any questions, he will consult Brother Zhang and learn a lot.

Like the speed control of the centrifuge, the suction and discharge control of the pipette, etc., these sloppy recovery operations will affect the cell survival rate and proliferation rate.

Finally, Zhang Lin poured the cell suspension into the petri dish, added an appropriate amount of culture solution, and put it into a 37 ° C, 5% CO2 cell incubator.

"Wait, at least two days." Zhang Lin took off his mask and pushed his glasses. "I suggest you keep it for a week, so that the rate of tumor formation is guaranteed."

"Take a batch for two days." Gu Jun said with a shrug. There is no way. The time is not enough. The system shows that he will get worse after 30 days. If it is true, it may be difficult to get out of bed and walk. He said again: "Senior brother, go out for a meal at night? I'll treat you."

"No." Zhang Lin waved his hand, "I will go to feed the mice at night, and I will treat you."

……

For the next two days, Gu Jun spent on campus. In addition to gathering old with friends, the first thing is to read a book to study and prepare for the make-up exam. It is also very troublesome to repeat the grade if you ca n’t die; the second is to go every day. The experiment building takes care of the cells and replaces the culture medium once.

On the morning of the third day, Zhang Lin personally looked at it and said that the cells had grown barely. He helped to subculture and prepare cell suspensions.

And Gu Jun went to the school's experimental animal center, which is actually a large breeding base, located a few hundred meters outside the school campus, very lonely, probably because it is permeated with the smell of various animals.

He bought five **** mice, each of which was more than one hundred pieces, which was quite expensive. Fortunately, he still had tens of thousands of deposits.

Each of these **** mice has the size of two or three fingers and no hair. They are packed in a sterile box. Once they come into contact with the environment of bacteria, they are easily infected and die. Therefore, the usual feeding and experimenting processes need to be carried out in a barrier environment.

Dongda's Barrier Environment Laboratory is located in the Laboratory Animal Research Center. This five-story yellow brick building stands next to the experimental building.

Gu Jun and Zhang Lin met in the lobby on the first floor of the research center and booked a small **** mouse room for one hour. This is a small room of only 21 square meters, divided into the front room and the back room, the front room has experimental benches and instruments, and the rear room is the rearing room.

Before entering, they and all the items they carry must undergo strict ultraviolet sterilization. The two set foot on the **** mouse room with a full-length protective clothing, masks, gloves, foot covers and other special equipment.

Quickly, on the anterior laboratory bench, Gu Jun marked five **** mice with a punch method, and then injected them with tumor cells subcutaneously on the right side of the buttocks.

Zhang Lin is in charge of assisting them. These cells hit humans, in theory, they can also form tumors. Seeing that Tu Haojun was so enthusiasm for the **** mice, he was sure that the little brother did not want to play murder.

After all five **** mice were injected, Gu Jun put them in the feeding cage, and then it was up to them to see if they could form tumors.

"Brother, thank you very much." Gu Jun gave Brother Zhang a compliment, and without Brother's help, it would definitely not be so smooth.

"The old Chinese doctor you said ..." Zhang Lin still looked at Conan with a vigilant look, "Is it really a liar?"

……

A week after, Gu Jun continued his previous life rhythm, but just went to the **** mouse room every day to see the caged **** mouse. Their breeding is under the unified care of the management staff of the **** mouse room, and each room and board costs 3 yuan per day.

He only completed three ordinary tasks this week, because those who need to cure and save people can't do it. Although the hospital is nearby, he is not a doctor. The tasks he completed were all related to medical exercises. There were already 5 boxes of pills in hand, and the proficiency of Tairan's hand reached the first weight (3000/5000).

On the seventh day, Gu Jun walked into the **** mouse room with sterile equipment again and observed the **** mice one by one.

They were not dead. A soy-like round tumor on the right side of the hip of No. 1 had grown and measured 0.5 cm in diameter; No. 3 and No. 4 also had obvious protrusions with a diameter of 0.3 cm and 0.5cm; No. 2 and No. 5 still showed no signs of tumor formation.

At this point he can be sure that five **** mice and three tumors.

Gu Jun set two tumor-forming **** mice as group A, one tumor-forming **** mouse as group B, two non-tumor **** mice were divided into group C and group D, and went to the animal center to buy 10 healthy **** mice , 5 set E group, 5 set F group. Groups A, C, and E will all receive the same dose of "different medicine" (his name) every day; while groups B, D, and F will not have any medications and are used for control purposes only.

Is tumor-bearing **** mice, non-tumor-bearing **** mice and healthy **** mice, each of which has a group of medication, and a group of medication.

At this time, six cages had been divided on the experimental platform, and the **** mice chirped and were busy.

Gu Jun is messing with 25 xenobiotic capsules. Since the system says that a box can extend the life of 5 days and a box of 5 capsules, it should be one capsule a day. This is the dose for adults, and the average weight of these **** mice is less than 20g. If they also take one capsule a day, it is estimated that they will be sacrificed immediately.

He poured out all the powder in a capsule and weighed the dose. Then he converted the dose of the **** mouse to his microgram according to his weight.

Soon, Gu Jun held a gastric-filled needle with a potion in one hand, grasped a healthy **** mouse in one hand, and began the first intragastric administration.

"Whee." The **** mouse struggled a little, but this poor thing was still pushed by him with this injection to make its contribution to medicine.

At this moment, Gu Jun's heartbeat was a little fast, his eyes blinked at the **** mouse in his hand.

He has not done the toxicity test of foreign medicine until this moment. If it died abruptly, perhaps this experiment would be meaningless. And now, he stared at it for five minutes, then 10 minutes ...

Half an hour later, the **** mouse still showed no abnormalities.

"It seems that the foreign medicine has no obvious acute toxicity." Gu Junsong breathed out. "We need to observe the chronic toxicity."

He continued to administer the medicine to the next **** mouse. After taking 10 minutes to complete the administration of the other 7 **** mice in the three administration groups, he observed for another hour. They all showed no abnormal reaction, and they still called kinetic energy, could drink water and eat feed.

"Dear friends, let's live." Gu Jun whispered, pressing his sore head, his condition became more and more obvious, "Please."

……

On the second and third day, Gu Jun continued to work in the **** mouse room every day.

The tumors of the **** mice in group B that have not been administered have increased from 0.3 cm to 0.6 cm, and their body weight and mental state have not changed; while the two **** mice in group A who have been administered have the tumor size after two days. No change, maintained at 0.5cm.

Gu Jun knows what this might mean, and the xenobiotics are really effective! These capsules can significantly inhibit tumor growth.

And the other administration of **** mice as usual, UU reading www.uukanshu.com This also means that the foreign medicine seems to have no adverse reactions and does not damage the body's function much.

"Maybe, this time it really saved?" He thought, although the restlessness was still entangled in his heart, a new hope burned at the same time.

The fourth day, the fifth day ... In a blink of an eye, a week passed.

The tumor of group B **** mice has grown to 1.5cm. The huge tumor is in sharp contrast with its thin body. It has started to lose weight and its spirit has become bad. However, the two **** mice in group A continued to be in stable condition, and all the **** mice administered were alive and well, which was no different from the blank control group.

Has to say that the results of this experiment are too satisfying.

The efficacy of the xenobiotic is better than the top tumor-targeted small molecule drugs currently on the market.

But Gu Jun also knows that the adverse reactions of foreign medicines cannot be completely ruled out, this just proves that it has no side effects in a short time. And this is an animal experiment after all, and there are certain differences from human clinical experiments.

However, the results of this experiment are already worth his risk.

This afternoon, after writing his suicide note, Gu Jun came to the waiting area of ​​the emergency department of Dongda Affiliated Hospital and sat down in a rare waiting seat. Surrounded by crowds of patients and family members, mostly crying children and coaxing parents, nurses hurriedly walked around, nurses pushed the rescue bed ...

Noisy, the emergency department broadcasting continued to call: "No. 182, Chen Ziyang. No. 183, Wang Yuxuan. No. 184, Huang Zixuan ..."

Took a breath, Gu Jun decided to throw a capsule of xenobiotic into his mouth and swallowed it with a sip of mineral water.

"Come on." He looked calmly and looked at the emergency sign on the wall over there. "If there is any toxic reaction, come all."