Plague Doctor

Cai Zixuan looked around and looked forward, before saying, "The leader of our group is Wang Ruoxiang."

"This is the case?" Gu Jun frowned helplessly, and thought it was something, "I'm not surprised at all, I'm not surprised that she's not her."

"Yeah, that's Wang Ruoxiang." Cai Zixuan said with emotion.

Wang Ruoxiang is the monitor of their squad, with strong ability, beautiful people and good personality. Such a goddess of learning, and her pursuit of course is from the teaching building to the bureau of the bureau. Tuhaojun is one of them. It was a vigorous pursuit, but eventually refused to end with Wang Ruoxiang's stern words: "Sorry, we are not one Humanoid, I would not like people like you. "

Eh, a tall and handsome man who is rich, poor, or an eight-year man, is this kind of person?

Everyone said at that time, thank Tu Haojun and squad leader, let them believe in love again.

"The squad leader is still single now." Cai Zixuan obviously didn't know how bad-looking he was now.

"Don't look at me like this." Gu Jun laughed at himself. "It's all gone." Indeed, Tu Haojun's pursuit of the squad leader is somewhat like, and a little bit wants to catch everyone's goddess. Those profound things don't matter.

"Is it." Cai Zixuan stopped talking about the monitor, and looked at his watch. "Hey, it's almost 8 o'clock. I have to go to the laboratory."

"Let's go, I also want to go to the experiment building." Gu Jun said.

Cai Zixuan suddenly showed his complacent eyes, and he just said that he was not worried about the squad leader, so he would turn around and join in the excitement. It ’s nice to be young.

"What?" Gu Jun was really helpless. I wanted to explain and I didn't know where to start. "Forget it ..."

"Haijun, you don't need to say, understand, once the sea is difficult to water, I understand."

"You know a sponge."

Medical experiment building is in the northwest of the campus. It is a ten-story white-wall brick building with little appearance, and even across the door is a badly worn out cement floor. But there are well-equipped laboratories with various instruments in the building for teachers and students to use for scientific research.

"This way." After entering the building, Cai Zixuan walked in front, and introduced the situation to Gu Jun while walking.

The subject of the experiment conducted by their group is called "Studies on Photodynamic Therapy of Tumors with Several Photosensitizers". It is a study in which tumors are implanted subcutaneously in mice, then photodynamic therapy is performed with different photosensitizers, and then the changes in the tumor are observed .

This time the funds allocated are only 30,000 yuan, which is too much for an undergraduate research experiment, but an imported pure Photofrin photosensitizer can cost tens of thousands. In order to be able to make several more photosensitizers, Wang Ruoxiang made various calculations to save money and set up a domestically-made Photofrin group, doteporfin group, group, plus a blank control group, a total of 5 groups of mice.

"The research plan and the opening report are all made by the team leader." Cai Zixuan couldn't help but sigh, "It's really Wang Ruoxiang."

Although Professor Gu is a tutor, he will only occasionally take the time to guide him. It is his business to score high SCI articles like a big cow like his old man. Therefore, the Pioneer Cup still depends on the students themselves. The team leader does the most things, but it is an opportunity everyone wants.

Because if the experiment produces a paper, Wang Ruoxiang is the first author, Cai Zixuan they can only be behind.

"We are still in the first stage, which is to establish an ascites tumor-bearing mouse model. It has been cultured for a week, and today it is not successful."

Cai Zixuan said a big pass, and suddenly found that Gu Jun had not spoken for a while, and he frowned as if he heard the word tumor ...

"Uh, moojun ... you know, the success rate of modeling is not 100%."

Cai Zixuan scratched his front baldness. I really didn't know what he just said. Gu Jun understood it.

"What else in the world can be 100%." ​​Gu Jun lowered his head to cover his face, friend, I haven't learned how to get that part.

"Really." Cai Zixuan was touched by this sentence, and his face was filled with vicissitudes. "Everything works, like a dream bubble."

Okay, Gu Jun can't understand ...

In the conversation, the two took the elevator to the eighth floor, and the laboratory used by their team was on this floor.

Walked along the corridor, and outside the door of the laboratory, Cai Zixuan opened the door and walked in first. When the team members arrived, he became the last one.

"Zixuan, just waiting for you."

"what?"

At this time, when everyone saw a person who came in later, the laboratory suddenly calmed down, leaving only the chirping of the mouse.

Tuhaojun ...?

Did n’t he drop out of school to travel around the world? Did n’t he say he hung up? Not that he ...

"Hello everyone." Gu Jun smiled and waved to everyone, greeting Wang Ruoxiang, "Hello squad leader."

Wang Ruoxiang was standing by an experimental bench. Her black medium to short hair, the delicate melon seed face was pretty but plain looking, and her long figure was wearing a white coat. Wang Ruoxiang saw him, slightly frowned, "Hello Gu Jun." The voice was also very nice.

Gu Jun nodded to her, and after three months in Shibei, the monitor was still so beautiful, but he was no longer in the past.

"Haojun had something to suspend before school, and now he is back." Cai Zixuan explained to everyone, and introduced to Gu Jun: Clinical student Xu Hai is an acquaintance, and Zhang Haoran in basic medicine, He Yuhan in pharmacy, a group of 5 Everyone is there.

Cai Zixuan asked: "Squad leader, Hao Jun wants to take a look with us, won't it bother you?"

Wang Ruoxiang pursed her lips in silence, only to say: "Stand aside and don't do anything."

Local tyrants. She murmured in her heart, remembering that she had had a superficial affection for him at the beginning of school a few years ago ... vomiting. This kind of person is delicious and lazy, with all the five poisons, the dead wood cannot be carved, and the mud can't help the wall ... the mice are better! It may only be contributed to medicine by being pulled out for slice research.

"No." Gu Jun stood aside obediently, but always felt Wang Ruoxiang's eyes were not good ... illusion.

There are already a row of mouse feeding cages on the experimental table, and there are a group of white Kunming mice in each cage. This is the most commonly used experimental animal. It is very docile and small. It weighs only 18-22g at 1-1.5 months of age and is less than 10cm in length. It is available for experimentation.

Except for a cage of model mice, the rest are all blank mice, which Wang Ruoxiang just bought from the school's Experimental Animal Center. Once the modeling is successful, they can come in handy.

The team members have arrived, and everyone started to work immediately.

Crackling! Wang Ruoxiang put on rubber gloves and went to the mouse cage of the model mouse to catch the mice. These mice were numbered with the staining method. She caught the mouse No. 1. Cai Zixuan and several others were in charge of assistance, recording, video recording, and other work.

"The eighth day of S180 mouse fibrosarcoma implanted in the abdomen." Wang Ruoxiang's expression was focused and serious. He gently pressed the abdomen of the No. 1 mouse, which obviously bulged, and the mouse issued a painful chirp. She struggled, but she was firmly in her hands.

She said: "The ascites in No. 1 mouse continues to grow, and it is already a frog's abdomen.

Hearing what she said, the team members were all a little excited, and they all gathered together to see more clearly. "Is this the" frog belly "." "Great."

"It's worthy of a frog's belly, and his belly is really big." Cai Zixuan said with emotion.

Although they are all eight-year programs, it is the first time to do this kind of real scientific research experiment.

"Oh." Gu Jun also leaned over to see, still understand what they are doing.

The first reason to make ascites tumor-bearing mice is to obtain a large number of highly active S180 tumor cells. To know that the original cell line is very expensive, one tube can only inject one mouse, and the activity is very fragile, and the four treatment groups they set up need to cultivate hundreds of mice.

Therefore, it is to manufacture first and then expand. Now the mouse grows out of the frog's abdomen and can "absorb ascites".

"Gu Jun." Wang Ruoxiang suddenly turned his head and glanced at him. "Can you go away? It's in the way."

"Yes." Gu Jun shrugged and walked away. Your aura is okay, even one meter away from you.

Cai Zixuan did not see them all, Wang Ruoxiang continued to the next step.

She used a 5ml syringe to pierce the abdominal cavity of No. 1 mouse. A tube of yellow clear ascites was successfully drawn out and injected into an Eppendorf cone bottom centrifuge tube. After centrifugation, you can immediately see a large amount of white precipitate appearing on the bottom of the centrifuge tube. That is the tumor cells.

"Mice No. 1 was successfully modeled." Wang Ruoxiang was relieved and smiled to the team members: "There are a lot of S180 tumor cells in ascites!"

"Yeah!" "Haha." These are young people, nobody cares about the feelings of mice, they cheered, Cai Zixuan praised: "Take the first step to success!"

The first step was successful, and then these cells were given to the mice in the treatment group to make subcutaneous tumors, and then the tumor was treated with photodynamic therapy.

There are still many things to do. They continue to work hard, remove the supernatant from the centrifuge tube, resuspend the count, adjust the cell concentration ...

In order to maintain cell activity, all injections should be completed within one hour. So they are busy with a modeling mouse, UU reading www. uukanshu.com to the next one.

"Adjust the cell concentration to 1 × 10 ^ 7 / ml ......"

"Yuhan, you are responsible for taking 0.2ml cells per needle."

"Team leader, the anesthetic is ready!"

Everyone of them is fighting with high spirits, and no one can care about what the local tyrant has done. He even forgot to stand and watch.

Gu Jun looked at himself quietly, watching He Yuhan with cell suspension, Cai Zixuan anesthetized healthy mice with pentobarbital sodium, Xu Hai and Zhang Haoran then marked the mice with number and hip hair removal, and finally given by Wang Ruoxiang Tumor cells were injected subcutaneously on the right hip of the mouse ...

No. 1, No. 2, No. 3, one after another.

Suddenly, Gu Jun's head cracked, was it a brain stem tumor attack ...

The pain increased sharply, and he could not help crying: "Ah!" The scene in front of him became trance, flickering like the shadow of a nightmare.

He seemed to be unable to breathe air. He seemed to be in a ruined laboratory. Centrifuges, microscopes, sterilizers and other instruments fell to the ground. Broken test tubes, messy documents, blurred photo frames, and rotten animals. The corpse ... The dust turned all this into a gray color, without a breath of life, only chaos and silence.

The pain in the brain is getting worse, but the scene is becoming clearer. He can see some unspeakable instruments.

There are also the twisted corpses on the ground ... I can't recognize what animal ...

He saw again that there was a line of blood painted on the wall inside, and it was the mysterious language on the pill box, but compared to the standard of the printed type, the strokes of the line of blood were all kind of scary. weird.

In a trance, Gu Jun suddenly seemed to understand the meaning of the blood.

He murmured.